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Table 2 Immunological differences between arthritic and non-arthritic animals as well as mice with or without spondylitis

From: BALB/c mice genetically susceptible to proteoglycan-induced arthritis and spondylitis show colony-dependent differences in disease penetrance

Measured parameters Arthritic animals
(n = 154)
Non-arthritic animals
(n = 24)
Mice with spondylitis
(n = 80)
Mice without spondylitis
(n = 47)
In vitro T-cell proliferation, SI 2.96 ± 0.07 3.25 ± 0.13 3.00 ± 0.09 3.00 ± 0.14
In vitro IL-2 production (CTLL-2), SI 2.67 ± 0.07 2.73 ± 0.15 2.68 ± 0.10 2.66 ± 0.11
In vitro IL-4 production, ng/106 cells 2.65 ± 0.14 2.91 ± 0.34 2.79 ± 0.22 2.39 ± 0.18
In vitro IL-6 production, ng/106 cells 1.83 ± 0.01 2.17 ± 0.33 1.88 ± 0.14 1.86 ± 0.19
In vitro IFN-γ production, ng/106 cells 8.06 ± 0.28 8.50 ± 1.07 7.61 ± 0.37 9.56 ± 0.65a
In vitro TNF-α production, ng/106 cells 0.74 ± 0.01 0.77 ± 0.02 0.74 ± 0.01 0.75 ± 0.02
Serum IL-4, pg/mL 15.29 ± 2.41 22.80 ± 5.92 15.52 ± 3.32 18.49 ± 4.46
Serum IL-6, pg/mL 166.45 ± 20.21 106.18 ± 26.97 97.59 ± 14.94 65.05 ± 20.61
Serum IL-1β, pg/mL 96.36 ± 11.55 41.70 ± 11.29 163.78 ± 27.72 143.10 ± 31.16
Serum IFN-γ, pg/mL 26.14 ± 2.62 34.59 ± 6.27 25.05 ± 3.65 33.49 ± 4.55
IgG1 antibodies to human PG, mg/mL 12.75 ± 0.57b 8.30 ± 0.94 23.33 ± 0.78b 8.85 ± 0.86
IgG2a antibodies to human PG, mg/mL 1.36 ± 0.14a 0.72 ± 0.29 1.44 ± 0.21a 0.82 ± 0.17
IgG1 antibodies to PG, μg/mL 172.55 ± 13.47b 76.41 ± 10.56 174.99 ± 18.95a 116.68 ± 17.7
IgG2a antibodies to PG, μg/mL 68.01 ± 5.68b 24.79 ± 6.76 72.10 ± 8.18b 34.57 ± 6.16
  1. All animals were immunized simultaneously with human cartilage proteoglycan in dimethyldioctadecyl-ammonium bromide. A total of three injections were given at 3-week intervals, and mice were sacrificed 3 weeks after the third injection (on days 63 and 64) when all in vitro assays were performed. Values represent mean ± standard error of the mean. Histological analysis was performed, and each inflamed intervertebral disc (IVD) was scored from '0' to '8' as described in Materials and methods. Positive (spondyloarthopathic) animals were combined if at least one IVD was affected with inflammation. Superscript letters indicate the level of significance (aP < 0.05 and bP < 0.01) between arthritic and non-arthritic animals or between mice with or without spondylitis. IFN-γ, interferon-gamma; IL, interleukin; PG, proteoglycan; SI, stimulation index; TNF-α, tumor necrosis factor-alpha.