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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Candidate autoantigens identified by mass spectrometry in early rheumatoid arthritis are chaperones and citrullinated glycolytic enzymes

Figure 3

Two-dimensional gel electrophoresis maps of native and citrullinated rabbit aldolase. Two-dimensional gel electrophoresis maps of (a) native rabbit aldolase and (b) citrullinated rabbit aldolase. Mass spectra of (c) digested spot 1 and (d) digested spot 5. The isotope clusters correspond to the peptide RLQSIGTENTEENR with a mass of 1,646.809 Da for the peptide without post-translational modification and of 1,660.825 for the methylated peptide. The intensity increase observed for the second peak of the isotopic clusters in (d) is linked to the rate of acidification of the protein; this indicates the citrullination process. Beyond the fifth spot in (b), the rabbit aldolase is too citrullinated for the peptide RLQSIGTENTEENR to be seen after the trypsin digestion. m.w., molecular weight; pI, isoelectric point.

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Arthritis Research & Therapy

ISSN: 1478-6362

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