Figure 5
From: Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes
Induction of thymic stroma lymphopoietin (TSLP) and secretory leukocyte protease inhibitor (SLPI) synthesis by activated rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). TSLP (a) and SLPI (b) release was determined by enzyme-linked immunosorbent assay in supernatants of RA FLSs stimulated 48 hours (TSLP) and 72 hours (SLPI) with microparticles (MPs) (40 nM phosphatidylserine equivalents) isolated from RA synovial fluids, lipopolysaccharide (LPS)-treated THP-1 cells, and PHA, PMA and Adt-D (PPA)-treated CCRF-CEM cells. Data are expressed as the mean of triplicate samples ± standard deviation and are representative of three independent experiments. *P < 0.05. C, control medium; IFN-γ, interferon-gamma; SN, control supernatants; TMP, control Hanks' balanced saline solution.