Activation of extracellular-regulated kinase (ERK1/2) and nuclear factor κB (NFκB) following infection of synovial fibroblasts with Candida albicans. (a) Synovial fibroblasts were infected for 12 h with C. albicans and levels of total and phosphorylated ERK1/2 (P-ERK1/2) assessed. Tubulin served as protein loading control. Shown is a representative western blot from one of five experiments. (b) Synovial fibroblasts were infected for 6 h with C. albicans and NFκB activation assessed by electrophoretic-mobility shift assay (EMSA). Upper panel: NFκB EMSA. The first lane of the NFκB series is the blank control. The lower series is the cold control. Lower panel: semiquantitative analysis of EMSA band density (N = 3, *P < 0.05).