Figure 4

TNF-α-induced chromatin condensation and TP53INP1α expression. The effect of TNF-α (5 and 10 ng/ml) on both BALB/c and non obese diabetic (NOD) isolated acinar cells was determined by (a) TP53INP1α expression and (b) Hoechst staining as in Figure 1 after six hours incubation of isolated acini with the cytokine at 37°C with 5% carbon dioxide. Gel bands and apoptotic acini shown are representative of separate experiments with NOD and control cells. Results on percentage of apoptotic cells and TP53INP1 expression are means ± standard error of the mean of five and three experiments respectively. *P < 0.05 vs. basal values of NOD acini; **P < 0.01 vs 5 ng/ml TNF in BALB/c cells.