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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration

Figure 3

Effect of cytokine stimulation on tumour necrosis factor-alpha (TNF-α) and TNF receptor gene expression in normal or degenerate nucleus pulposus (NP) cells. Normal or degenerate NP cells were cultured in alginate pellets and stimulated by the addition of 10 ng/mL TNF-α (a) or IL-1β (b). Quantitative real-time polymerase chain reaction was used to analyse the effect of cytokine stimulation on TNF-α, TNF receptor 1 (TNF-R1) and TNF receptor 2 (TNF-R2) gene expression All samples are relative to the housekeeping gene GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and normalised back to untreated controls. Results are given as mean ± standard error of the mean (n = 3). *P ≤ 0.05 when compared with untreated controls.

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Arthritis Research & Therapy

ISSN: 1478-6362

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