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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-α and matrix metalloproteinase-13 in human chondrocytes

Figure 2

Human osteoarthritis chondrocytes in monolayer culture maintain their phenotype. Primary chondrocytes from osteoarthritis patients were cultured for 72 hours and were then split and cultured for an additional 3 days (passage 1). Expression of (a) type-2 collagen (COL2A1) and (b) aggrecan (ACAN), type-10 collagen (COL10A1) and SRY-box containing gene 9 (SOX-9) was determined by RT-PCR. M, 100 bp DNA ladder; P, positive control cDNA; P1, primary chondrocytes; P2, passage 1 chondrocytes.

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