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Table 1 Characterization of native BSA and AGE-BSA under identical experimental conditions

From: Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-α and matrix metalloproteinase-13 in human chondrocytes

Parameter

AGE-BSA

Native BSA (control)

A 340

0.625 ± 0.13*

0.228 ± 0.05

Fluo [ex 360/em 430 nm]

749.4 ± 19.5**

80.4 ± 4.8

Fluo [ex 330/em 395 nm]

754.6 ± 17.2**

83.2 ± 5.7

Fluo [ex 365/em 440 nm]

741.4 ± 28.1**

84.8 ± 5.2

Fluo [ex 485/em 530 nm]

734.1 ± 20.4**

85.1 ± 5.3

Fluo [ex 280/em 350 nm]

733.2 ± 19.1**

84.0 ± 4.4

  1. Data expressed as the mean ± standard deviation of 12 independent assays. A340, absorbance at 340 nm; AGE, advanced glycation end product; Fluo [ex/em], fluorescence [excitation/emission], Band width was set at 40/40 for fluorescence measurement. *P < 0.01 versus control, **P < 0.0001 versus control.
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Arthritis Research & Therapy

ISSN: 1478-6362

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