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Table 1 Characterization of native BSA and AGE-BSA under identical experimental conditions

From: Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-α and matrix metalloproteinase-13 in human chondrocytes

Parameter AGE-BSA Native BSA (control)
A 340 0.625 ± 0.13* 0.228 ± 0.05
Fluo [ex 360/em 430 nm] 749.4 ± 19.5** 80.4 ± 4.8
Fluo [ex 330/em 395 nm] 754.6 ± 17.2** 83.2 ± 5.7
Fluo [ex 365/em 440 nm] 741.4 ± 28.1** 84.8 ± 5.2
Fluo [ex 485/em 530 nm] 734.1 ± 20.4** 85.1 ± 5.3
Fluo [ex 280/em 350 nm] 733.2 ± 19.1** 84.0 ± 4.4
  1. Data expressed as the mean ± standard deviation of 12 independent assays. A340, absorbance at 340 nm; AGE, advanced glycation end product; Fluo [ex/em], fluorescence [excitation/emission], Band width was set at 40/40 for fluorescence measurement. *P < 0.01 versus control, **P < 0.0001 versus control.