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Table 1 Synthetic Toll-like receptor-9 agonists and antagonists used in the study

From: DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lprmice in vivo

  TLR9-antagonists Class Properties
4084-F CCT GGA TGG GAA B 5' linear, shortest active
INH-1 CCT GGA TGG GAA TTC CCA TCC AGG R 5' palindrome
INH-18 CCT GGA TGG GAA CTT ACC GCT GCA B 5' linear
INH-43 CCT GGA TAA AAA TTT TTA TCC AGG R Lacks GGG
INH-44 CCT GAA TAA AAA CTT ACC GCT GCA B Lacks GGG
INH-45 TAT GGA TGG GAA TTC CCA TCC ATA R Lacks CCT
INH-46 TAT GGA TGG GAA CTT ACC GCT GCA B Lacks CCT
INH-47 TAT GGA TTT TAA TTA AAA TCC ATA R Lacks CCT/GGG
INH-48 TAT GGA TTT TAA CTT ACC GCG GCA B Lacks CCT/GGG
5' OVHG-short CCT GGA TGG GAA TTC CCA TCC R 5' short overhang
(3 nucleotide OVHG)
5' OVHG-scr. short CCT GGA TGG GAA CTT ACC GCT B 5' linear
5' OVHG-medium CCT GGA TGG GAA TTC CCA R 5' medium overhang
(6 nucleotide OVHG)
5' OVHG-scr. medium CCT GGA TGG GAA CTT ACC B 5' linear
5' OVHG-long CCT GGA TGG GAA TTC R 5' long overhang
(9 nucleotide OVHG)
5' OVHG-scr. long CCT GGA TGG GAA CTT B 5' linear
INH-4
3' palindrome
TTC CCA TCC AGG CCT GGA TGG GAA R 3' palindrome
INH-13
3' scr. pal.
CTT ACC GCT GCA CCT GGA TGG GAA B 3' linear
3' OVHG-short CCA TCC AGG CCT GGA TGG GAA R 3' short overhang
(3 nucleotide OVHG)
3' OVHG-scr. short ACC GCT GCA CCT GGA TGG GAA B 3' linear
3' OVHG-medium TCC AGG CCT GGA TGG GAA R 3' medium overhang
(6 nucleotide OVHG)
3' OVHG-scr. medium GCT GCA CCT GGA TGG GAA B 3' linear
3' OVHG-long AGG CCT GGA TGG GAA R 3' long overhang
(9 nucleotide OVHG)
3' OVHG-scr. long GCA CCT GGA TGG GAA B 3' linear
4173 RRR RRR RRR RRR RRR B Linear control, R = random
  TLR9-agonists Class Properties
CpG-2336 ggGGACGACGTCGTGgggggg A(D) Lowercase PS linkages
CpG-1826 TCC ATG ACG TTC CTG ACG TT B(K) Linear, murine
CpG-2006 TCG TCG TTT TGT CGT TTT GTC GTT B(K) Linear, human
  1. OVHG, overhang; pal., palindrome; PS, phosphorothioate; scr., scrambled; TLR, Toll-like receptor.