Modulation of glucose transporter-1 protein content by different extracellular glucose concentrations. Glucose transporter-1 (GLUT-1) protein normalized to the respective actin band in chondrocytes cultured in media with 0 mM, 10 mM (regular glucose medium (RGM)) or 30 mM glucose (high glucose medium (HGM)) for 18 or 48 hours. (a) Normal chondrocytes (n = 4). (b) Osteoarthritis chondrocytes (n = 6). Results expressed as the percentage relative to the respective control cells maintained in RGM. **P < 0.01 and ***P < 0.001 relative to cells maintained in RGM. Bars = mean ± standard deviation.