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Table 2 Technical performance of the KS capture OA-1 ARGS ELISA

From: Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: a cross-sectional study

Linear measuring range of standard 0.02 to 1 pmol/ml
Minimal dilution of SF 1:50
Minimal detectable concentration in neat SF 1 pmol/ml SF
Intra assay CV (n = 10) 6.1%
*Inter assay, intra lot CV (n = 5, 1st lot) 12.2%
*Inter assay, intra lot CV (n = 23, 2nd lot) 15.6%
Inter assay, inter lot CV (n = 28) 19.7%
Dilution of SF 1:50 1:400 1:800 1:1600
Spiking recovery (mean; range) 116%;
109 to 121%
93%;
75 to 104%
81%;
75 to 88%
104%;
98 to 113%
  1. * Between assay variation in lot numbers (1st, #5L21/1; 2nd, #7F25/1) of the KS capture plates from Biosource.
  2. CV = coefficient of variation; KS = keratan sulfate; SF = synovial fluid.