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Table 2 Technical performance of the KS capture OA-1 ARGS ELISA

From: Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: a cross-sectional study

Linear measuring range of standard

0.02 to 1 pmol/ml

Minimal dilution of SF

1:50

Minimal detectable concentration in neat SF

1 pmol/ml SF

Intra assay CV (n = 10)

6.1%

*Inter assay, intra lot CV (n = 5, 1st lot)

12.2%

*Inter assay, intra lot CV (n = 23, 2nd lot)

15.6%

Inter assay, inter lot CV (n = 28)

19.7%

Dilution of SF

1:50

1:400

1:800

1:1600

Spiking recovery (mean; range)

116%;

109 to 121%

93%;

75 to 104%

81%;

75 to 88%

104%;

98 to 113%

  1. * Between assay variation in lot numbers (1st, #5L21/1; 2nd, #7F25/1) of the KS capture plates from Biosource.
  2. CV = coefficient of variation; KS = keratan sulfate; SF = synovial fluid.