Skip to main content

Advertisement

Table 2 Absolute number of circulating CD3+ T cells, CD4+ T cells, CD8+ T cells, percentage ofactivated CD4+ T cells, regulatory CD4+ T cells (Treg) and percentage of cells expressing intracellular CTLA-4 in healthy controls, patients with Spondylarthropathies and rheumatoid arthritis

From: Increased production of soluble CTLA-4 in patients with spondylarthropathies correlates with disease activity

  Healthy controls
(n = 15)
Spondylarthropathies
(n = 32)
Rheumatoid arthritis
(n = 22)
P
CD3+ T cells (/mm 3 ) 1397 ± 79
(925 to 1806)
1360 ± 80.8
(622 to 2681)
1129 ± 140
(336 to 3024)
0.005 (KW)
RA vs HC 0.004 (MW)
SpA vs HC NS
SpA vs RA 0.007 (MW)
CD4+ T cells (/mm 3 ) 817 ± 45
(449 to 1024)
875 ± 49.5
(391 to 1699)
804 ± 102
(239 to 2306)
NS
CD8+ T cells (/mm 3 ) 573 ± 47
(379 to 860)
457 ± 39.6
(130 to 979)
326 ± 52
(72 to 1260)
0.0006 (KW)
RA vs HC 0.0007 (MW)
SpA vs HC 0.051
RA vs SpA 0.006 (MW)
HLA-DR+ Foxp3-
CD4+ T cells
(% of CD4+ T cells)
16.6 ± 5.53
(5 to 70)
22.03 ± 4.56
(4 to 100)
12.14 ± 1.29
(5 to 26)
NS
CD25+ Foxp3+
CD4+ T cells
(% of CD4+T cells)
7.94 ± 1.04
(3.6 to 16)
8.2 ± 0.61
(4 to 14.4)
8.72 ± 0.7
(2.9 to 13.5)
NS
Intracellular CTLA-4 in CD4+ T cells (%) 4.35 ± 1.10
(1 to 13.3)
2.51 ± 0.40
(0 to 5.9)
1.35 ± 0.48*,§
(0 to 7.5)
0.005 (KW)
RA vs HC 0.003
SpA vs HC NS
RA vs SpA 0.02
Intracellular CTLA-4 in CD4+ Treg (%) 2.76 ± 0.44
(0.5 to 6.3)
1.88 ± 0.34
(0 to 5)
0.90 ± 0.32*,§
(0 to 3.9)
0.007 (KW)
RA vs HC 0.0045
SpA vs HC NS
SpA vs RA 0.027
Intracellular CTLA-4 in Activated CD4+ T cells (%) 1.59 ± 0.72
(0 to 7)
0.63 ± 0.26
(0 to 5)
0.5 ± 0.29
(0 to 5)
NS
Intracellular CTLA-4 in CD8+ T cells (%) 2.03 ± 0.57
(0.4 to 7)
2.64 ± 0.32
(0 to 6.3)
3.49 ± 0.88
(0.1 to 13.7)
NS
Intracellular CTLA-4 in CD3- lymphocytes (%) 0.89 ± 0.72
(0 to 8)
0.67 ± 0.21
(0 to 3.3)
3.50 ± 0.77*,§
(0 to 11)
0.0025 (KW)
RA vs HC 0.0077
SpA vs HC NS
SpA vs RA: 0.0017
Intracellular CTLA-4 in the lymphocyte gate (total lymphocytes) (%) 7.27 ± 1.27
(1.4 to 15)
3 ± 0.48
(0 to 7.6)
6.89 ± 1.3§
(0.1 to 19.3)
0.004 (KW)
RA vs HC 0.0045
SpA vs HC NS
SpA vs RA 0.007
  1. Percentage of intracellular CTLA-4 was determined by flow cytometry. Results are expressed as mean ± standard error of the mean, (range). The Kruskal-Wallis (KW) test was used to compare T cell subsets and intracellular CTLA-4 expression in the three groups. Then, Mann-Whitney U-test was used to calculate the exact P values between two groups.
  2. HC = healthy controls; HLA = Histocompatibility Leukocyte Antigen; KW = Kruskal-Wallis; MW = Mann-Whitney; NS = not significant; RA = rheumatoid arthritis; SpA = spondylarthropathie.