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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Effect of interleukin-1β on spinal cord nociceptive transmission of normal and monoarthritic rats after disruption of glial function

Figure 3

Effect of IL-1β on spinal cord wind-up activity in propentofylline- and saline-treated normal and monoarthritic rats (NS, MS, NP and MP groups). (a) Time course of wind-up scores (% change) 10, 20 and 40 minutes after administration of saline intrathecally. One-way analysis of variance (ANOVA) did not detect significant intra-group changes in either group after intrathecal saline. The arrow indicates injection of saline at zero time. Values are means ± standard error of the mean (SEM). n = 8 rats in all groups. (b) Time course of wind-up scores (% change) 10, 20 and 40 minutes after administration of 2 ng IL-1β intrathecally. The arrow indicates injection of IL-1β at zero time. Values are means ± SEM. n = 8 rats in all groups. Values are means ± SEM. n = 8 rats in all groups. Intra-group analyzes by one-way ANOVA detected significant wind-up increases in the NS and MS groups after intrathecal IL-1β (NS group: P ANOVA = 0.0403, F = 3.154; MS group: P ANOVA < 0.0004, F = 8.363), and significant wind-up decreases in the NP and MP groups after intrathecal IL-1β (NP group: P ANOVA = 0.0407, F = 3.147; MP group: P ANOVA = 0.0135, F = 4.253). Significant changes after IL-1β administration are denoted by the asterisk (*P < 0.05, Dunnett post hoc test). (c) Global effect of saline intrathecally on C-reflex wind-up activity on the 40-minute period of testing, as revealed by percent change of area under the curves (AUCs). Values are means ± SEM. n = 8 rats in all groups. Two-way ANOVA detected that neither the propentofylline-treatment, nor the monoarthritic condition, nor the combination of propentofylline-treatment and monoarthritis affected the AUC scores significantly or modified the response to saline intrathecally. (d) Global effect of 2 ng IL-1β intrathecally on C-reflex wind-up activity on the 40-minute period of testing, as revealed by percent change of AUCs. Values are means ± SEM. n = 8 rats in all groups. Two-way ANOVA identified the propentofylline treatment (P ANOVA < 0.0001, F = 46.91), but not the monoarthritic condition (P ANOVA = 0.5799, F = 0.31), as a factor influencing the effect of IL-1β on wind-up activity. # indicates statistically significant difference (P < 0.01, Bonferroni post hoc test) when comparing propentofylline-treated animals (NP and MP) against the respective saline-treated animals (NS and MS). NP = normal rats receiving intrathecal propentofylline; NS = normal rats receiving intrathecal saline; MP = monoarthritic rats receiving intrathecal propentofylline; MS = monoarthritic rats receiving intrathecal saline.

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