Figure 4

Identification of TIARP-expressing cells in splenocytes and joints of arthritic mice. (a) Splenocytes were isolated from naïve (day 0) mice and mice with glucose-6-phosphate isomerase (GPI)-induced arthritis and then were separated into four groups (CD4⁺, CD19⁺, CD11b⁺, and CD11c⁺) by magnetic-activated cell sorting. The expression of TIARP mRNA was analyzed by quantitative real-time polymerase chain reaction at days 0 and 7. TIARP mRNA was expressed mainly on CD11b⁺ cells in arthritic mice. Data are mean ± standard error of the mean of five mice per group. *P < 0.05 (Mann-Whitney U test). (b) Joints were obtained from mice with GPI-induced arthritis on day 14 and stained with anti-TIARP antibodies (top panels) and control antibodies (bottom panels). Inflamed synovial tissue of arthritic mice was stained with anti-TIARP antibodies. GAPDH, glyceraldehydes-3-phosphate dehydrogenase; TIARP, tumor necrosis factor alpha-induced adipose-related protein.