Suppressive effect of secretory phospholipase A2inhibitory peptide on interleukin-1β-induced matrix metalloproteinase production in rheumatoid synovial fibroblasts, and its antiarthritic activity in hTNFtg mice

Table 1 Percentage of fibroblasts and contaminating cells in primary cultures of RA and OA synovial fibroblast cells at various passages

Passage	Cell type	% positive cells (Mean ± SEM)*
		RA SF	OA SF
First	Fibroblast (Prolyl-4-hydroxylase +)¹	75 ± 8.0	68 ± 5.0
	Monocyte/macrophage (CD14+)²	15 ± 2.0	21 ± 3.5
	T-cells (CD3+)³	0.8 ± 0.2	1.2 ± 0.3
	B-cells (CD20+)⁴	0.9 ± 0.3	0.8 ± 0.2
Third	Fibroblast (Prolyl-4-hydroxylase +)	99 ± 0.5	98.5 ± 0.6
	Monocyte/macrophage (CD14+)	0.8 ± 0.2	0.6 ± 0.1
	T-cells (CD3+)	0.5 ± 0.1	0.8 ± 0.2
	B-cells (CD20+)	0.6 ± 0.2	0.5 ± 0.1
Fourth	Fibroblast (Prolyl-4-hydroxylase +)	98 ± 0.4	99.2 ± 0.4
	Monocyte/macrophage (CD14+)	1.0 ± 0.5	0.95 ± 0.3
	T-cells (CD3+)	0.5 ± 0.2	0.5 ± 0.1
	B-cells (CD20+)	0.9 ± 0.1	0.8 ± 0.1

* Total number = five rheumatoid arthritis (RA) and five osteoarthritis (OA) patients. Monoclonal antibodies used for flow cytometry: mAb 5B5 ¹; mAb Tyk4 ²; mAb UCHT-1 ³; mAb B-Ly1 ⁴. SEM = standard error of the mean; SF = synovial fluid.

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