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Table 1 Percentage of fibroblasts and contaminating cells in primary cultures of RA and OA synovial fibroblast cells at various passages

From: Suppressive effect of secretory phospholipase A2inhibitory peptide on interleukin-1β-induced matrix metalloproteinase production in rheumatoid synovial fibroblasts, and its antiarthritic activity in hTNFtg mice

Passage Cell type % positive cells (Mean ± SEM)*
   RA SF OA SF
First Fibroblast (Prolyl-4-hydroxylase +)1 75 ± 8.0 68 ± 5.0
  Monocyte/macrophage (CD14+)2 15 ± 2.0 21 ± 3.5
  T-cells (CD3+)3 0.8 ± 0.2 1.2 ± 0.3
  B-cells (CD20+)4 0.9 ± 0.3 0.8 ± 0.2
Third Fibroblast (Prolyl-4-hydroxylase +) 99 ± 0.5 98.5 ± 0.6
  Monocyte/macrophage (CD14+) 0.8 ± 0.2 0.6 ± 0.1
  T-cells (CD3+) 0.5 ± 0.1 0.8 ± 0.2
  B-cells (CD20+) 0.6 ± 0.2 0.5 ± 0.1
Fourth Fibroblast (Prolyl-4-hydroxylase +) 98 ± 0.4 99.2 ± 0.4
  Monocyte/macrophage (CD14+) 1.0 ± 0.5 0.95 ± 0.3
  T-cells (CD3+) 0.5 ± 0.2 0.5 ± 0.1
  B-cells (CD20+) 0.9 ± 0.1 0.8 ± 0.1
  1. * Total number = five rheumatoid arthritis (RA) and five osteoarthritis (OA) patients. Monoclonal antibodies used for flow cytometry: mAb 5B5 1; mAb Tyk4 2; mAb UCHT-1 3; mAb B-Ly1 4. SEM = standard error of the mean; SF = synovial fluid.