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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Hypertrophy is induced during the in vitrochondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer

Figure 3

Immunohistochemical analyses for cartilage matrix proteins of MSC pellets after chondrogenic induction with BMP-2 or BMP-4 gene transfer. Monolayer cultures of MSCs were infected with (a) Ad.GFP, (b) Ad.BMP-2 or (c) Ad.BMP-4 at 5 Ă— 102 vp/cell as indicated and placed into aggregate cultures. Immunohistochemical staining was performed on culture days 10 and 21 for collagen type II (left panels) and chondroitin-4-sulfate (right panels). Regions of positive immunostaining appear brown. Panels are reproduced at low (50Ă—; bar = 200 μm) or high (200Ă—; bar = 50 or 100 μm) magnification as indicated. Ad = adenoviral vector; BMP = bone morphogenetic protein; GFP = green fluorescent protein; MSC = mesenchymal stem cell.

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Arthritis Research & Therapy

ISSN: 1478-6362

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