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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Hypertrophy is induced during the in vitrochondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer

Figure 4

Histological and immunohistochemical analyses for hypertrophy of MSC pellets after chondrogenic induction with BMP-2 or BMP-4 gene transfer. Following genetic modification with (a) Ad.GFP, (b) Ad.BMP-2 or (c) Ad.BMP-4 aggregates after 10 and 21 days of culture stained for ALP (left panels) and collagen type X (right panels) are shown. Regions of positive immunostaining appear brown. Panels are reproduced at low (50Ă—; bar = 200 μm) or high (200Ă—; bar = 50 or 100 μm) magnification as indicated. Ad = adenoviral vector; ALP = alkaline phosphatase; BMP = bone morphogenetic protein; GFP = green fluorescent protein; MSC = mesenchymal stem cell.

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Arthritis Research & Therapy

ISSN: 1478-6362

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