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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis

Figure 3

Plasma blasts and Ig-secreting cells in synovial fluid (SF), peripheral blood (PB), and synovial tissue from juvenile idiopathic arthritis (JIA) patients. (a) Cells from JIA SF and PB, as well as from control PB, were analyzed with flow-cytometry gating, first on CD3-, CD14-, CD16-, and CD56- cells (non-B cell lineage cells), and then on CD19+cells, and finally analyzed for CD27 and CD20 expression. Results are expressed in a box plot as median percentage of positive cells, minimum and maximum value. *P < 0.05. One representative dot-blot for SF (middle panel) and PB (right panel) is shown. (b) IgG, IgA, or IgM CD19+immunoglobulin-secreting cells (ISCs) were detected in SF and PB from four JIA patients with ELISPOT. Results are expressed as mean ISC ± SD. *P = 0.028. (c) Serial synovial tissue sections from three JIA patients were stained with anti-IgG, anti-IgA, or anti-IgM mAbs by using the peroxidase method (brown staining).

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