Figure 4

Chemokine receptor and CD86 expression on switch memory B cells from juvenile idiopathic arthritis (JIA) patients. Cells from JIA synovial fluid (SF) and peripheral blood (PB) were analyzed with flow-cytometry gating first on CD3^-, CD14^-, CD16^-, and CD56^- cells (non-B cell lineage cells), then on CD19⁺cells, and subsequently on CD27⁺ or CD27^- cells before being analyzed for individual chemokines-receptor expression. (a) CC chemokine receptor expression on SF and PB CD27⁺switch memory B cells. Results are expressed in a box plot as median percentage of positive cells, minimum and maximum value. *** P = 0.0002; P = 0.0003. **P = 0.001; P = 0.004. *P = 0.019. (b) CXC chemokine receptor and CD86 expression on SF and PB CD27⁺switch memory B cells. Results are expressed in a box plot as median percentage of positive cells, minimum and maximum value. ***P = 0.0001. **P = 0.001; P = 0.002. (c) CCR expression on SF and paired PB CD27^- switch memory B cells. Results are expressed in a box plot as median percentage of positive cells, minimum and maximum value. ***P = 0.0001; P = 0.0003. **P = 0.0034; P = 0.0041. (d) CXCR and CD86 expression on SF and paired PB CD27^-switch memory B cells. Results are expressed in a box plot as median percentage of positive cells, minimum and maximum value. ***P = 0.0001; P = 0.0006. **P = 0.0011.