Figure 5
From: Melanocortin peptides inhibit urate crystal-induced activation of phagocytic cells
Effect of the caspase-1 inhibitor z-YVAD-fmk on inflammatory mediator production by monosodium urate (MSU)-stimulated human monocytes. Monocytes were incubated for 18 hours with MSU crystals (1 mg/mL) with or without z-YVAD-fmk (10 mM) or alpha-melanocyte-stimulating hormone (α-MSH) (10-6 M) or the two together. Interleukin (IL)-1β and IL-8 concentrations were measured in the cell-free supernatants. Means ± standard errors of the mean of three separate experiments are presented.