Figure 2From: Increased levels of circulating microparticles in primary Sjögren's syndrome, systemic lupus erythematosus and rheumatoid arthritis and relation with disease activityRepresentative flow cytometry density plots showing the gating protocol for microparticles. The gate of microparticles (MPs) was defined by use of Megamix containing fluorescent latex microbeads (0.5, 0.9 and 3 μm) (a) Quantitative estimation of MPs involved use of a fixed number of 3 μm microbeads, which were counted concomitantly with MP acquisition in the specific gate. ( b to d ) Gated MPs alone (b) without annexin V addition, (c) stained with annexin V FITC in a calcium-specific buffer, and (d) stained with annexin V in PBS (without calcium). (e) Isotype controls, (f) platelet MPs (CD61+), (g) leukocytes MPs (CD45+) using a single staining.Back to article page