Skip to main content
Figure 2 | Arthritis Research & Therapy

Figure 2

From: Increased levels of circulating microparticles in primary Sjögren's syndrome, systemic lupus erythematosus and rheumatoid arthritis and relation with disease activity

Figure 2

Representative flow cytometry density plots showing the gating protocol for microparticles. The gate of microparticles (MPs) was defined by use of Megamix containing fluorescent latex microbeads (0.5, 0.9 and 3 μm) (a) Quantitative estimation of MPs involved use of a fixed number of 3 μm microbeads, which were counted concomitantly with MP acquisition in the specific gate. ( b to d ) Gated MPs alone (b) without annexin V addition, (c) stained with annexin V FITC in a calcium-specific buffer, and (d) stained with annexin V in PBS (without calcium). (e) Isotype controls, (f) platelet MPs (CD61+), (g) leukocytes MPs (CD45+) using a single staining.

Back to article page