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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Regulation of pathogenic IL-17 responses in collagen-induced arthritis: roles of endogenous interferon-gamma and IL-4

Figure 1

Systemic and synovial IL-17 responses in collagen-induced arthritis. (a) Male DBA mice were immunized with chicken type II collagen and complete Freund's adjuvant (CFA) on day 0. Serum was collected serially by tail bleeds on days 0, 14, 28 and 42. IL-17 and IFN-γ were measured by ELISA in triplicate. Data is presented as composite mean of cytokine levels from 30 mice from two experiments. (b) Arthritis was assessed by clinical scoring of paw swelling and redness every other day from day 20. Mice with a clinical score of two in at least one paw were considered arthritic. IL-17/IFN-γ is the ratio of the absolute level of IL-17 in serum to the absolute level of IFN-γ in serum at day 28 after immunization with collagen and CFA. Data is presented as composite mean of 30 mice from two experiments. * mean significant with P = 0.01 and variance significant with P < 0.001. (c) Splenocytes and single cell suspension of draining inguinal lymph nodes, from day 28 after collagen and CFA immunization, were cultured for five days in the presence of collagen rechallenge. Supernatants were analyzed for IL-17 and IFN-γ by ELISA. Arthritis was scored as in materials and methods. Mice with a score of at least two in one paw were considered arthritic. Data is presented as composite mean of 30 mice from two experiments. * P < 0.05, ns = not significant. (d) Paws were collected from mice 28 to 32 days after collagen immunization. Both front and hind paws were cut into small pieces and cultured overnight in media. Supernatants were collected for measurement of IL-17, IL-4 and IFN-γ by ELISA. Data is presented as composite mean of 20 mice from two experiments. (e) Correlation analysis of paw IL-17 to paw IFN-γ and IL-4. Data is from arthritic paws. Non-arthritic paws did not have measurable quantities of IL-17.

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