Figure 2

Specimen Calc1. (a and b) Fourier Transform Infrared microspectroscopy in reflectance mode (FTIR-RM) protein and mineral maps, respectively, obtained from the whole specimen with 200 × 200 μm spatial resolution (SR). (c and d) FTIR-RM protein and mineral maps obtained from the black boxed regions in (a) and (b) with 80 × 80 μm SR. (e) FTIR-RM mineral map obtained from the cyan boxed region in (d) with 40 × 40 μm SR. Blue is the largest area under the related peaks and red is the lowest in the FTIR-RM maps. The individual size scale bars appear on the images and the maps. (f) Silicon drift detector energy dispersive X-ray spectrometry (SEM/SDD-EDS) Mg, Na, P and Ca maps of the green boxed region in (e). (g) SEM/SDD-EDS Mg, Na, P and Ca maps of the brown boxed region in (e). The size scale bars in all the SEM/SDD-EDS maps in (f) and (g) are the same as in (e) (1 mm). (h) SEM/SDD-EDS Ca map of the purple boxed region in (e) and in the Ca map in (g), obtained with higher magnification.