Specimen Calc8. (a) Bottom as received. (b and c) Cut face before and after freeze drying. (d) Visual map of the cut face after embedding and polishing. (e) Fourier Transform Infrared microspectroscopy in reflectance mode (FTIR-RM) protein maps obtained with (panel 1) 200 × 200 μm and (panel 2) 40 × 40 μm spatial resolution (SR). (f) FTIR-RM mineral maps obtained with (panel 1) 200 × 200 μm and (panel 2) 40 × 40 μm SR. Blue is the largest area under the related peaks and red is the lowest in the FTIR-RM maps. (g, panel 1) X-ray micro-computed tomography (μCT) 2D images obtained at various distances from the bottom of the embedded specimen. (panels 2 and 3) μCT 3D projection images rotated around the same plane as that mapped with the FTIR-RM. The individual size scale bars appear on the images and the maps.