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Table 1 The sequence of polymerase chain reaction primers used in this experiment

From: Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediators

Primer name Primer sequence Product size
MMP-1 sense 5'-CCT AGC TAC ACC TTC AGT GG-3' 338 bp
MMP-1 antisense 5'-GCC CAG TAC TTA TTC CCT TT-3'  
MMP-13 sense 5'-TTG AGG ATA CAG GCA AGA CT-3' 311 bp
MMP-13 antisense 5'-TGG AAG TAT TAC CCC AAA TG-3'  
VEGF sense 5'-ACT TCA GGC TCT TCT CCT TT-3' 288 bp
VEGF antisense 5'-TTC AGA CAA CCT GAG TCC TT-3'  
IL-6 sense 5'-CTG GTC TTT TGG AGT TTG AG-3' 344 bp
IL-6 antisense 5'-TTT CTG ACC AGA AGA AGG AA-3'  
IL-8 sense 5'-ACT TTC AGA GAC AGC AGA GC-3' 264 bp
IL-8 antisense 5'-GTG GTC CAC TCT CAA TCA CT-3'  
COX-2 sense 5'-TTC AAA TGA GAT TGT GGG AAA ATT GCT-3' 306 bp
COX-2 antisense 5'-AGA TCA TCT CTG CCT GAG TAT CTT-3'  
β-actin sense 5'-TCA TGA GGT AGT CAG TCA GG-3' 305 bp
β-actin antisense 5'-CTT CTA CAA TGA GCT GCG TG-3'  
  1. bp, base pairs; COX-2, cyclooxygenase-2; IL, interleukin; MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor.