Figure 4

Co-immunization with altered peptide ligands does not affect IL-17 production. Mice were immunized with 10 μg glucose-6-phosphate isomerase hGPI_325--339 and 50 μg each altered peptide ligand (APL). Draining lymph node cells on day 6 were stimulated for 24 hours in vitro (a) with 10 μM mouse GPI_325--339 or (b) with 10 μM corresponding APL. GoldiStop was added in the last 4 hours of each culture. Flow cytometry analysis for IL-17 and TCRVβ repertoire was gated in CD4^high cells. None, immunization with no APL (hGPI_325--339alone). Representative flow cytometry data of two independent experiments.