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Table 1 Summary of evaluated amplification methods, target on chlamydial genome, product size and references of primer sequences

From: Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems

 

Target

Product size

Primer sequences

Application in Germany

PCR 1

omp-1 (152 bp)

152 bp

Bobo and colleagues [24]

Kuipers and colleagues [17]

PCR 2

omp-1 (739 bp)

739 bp

Gérard and colleagues [27]

Freise and colleagues [9]

PCR 3

Plasmid

402 bp

Wilkinson and colleagues [23]

M. Rudwaleit, Benjamin Franklin Hsp., Berlin

PCR 4

16sRNA

141 bp

Bas and colleagues [18]

 

LCR

Plasmid

LCX®

Abbott

Kuipers and colleagues [17]

  1. LCR = ligase chain reaction; omp-1 = major outer membrane protein 1.