Skip to main content

Advertisement

Table 3 Sensitivity of PCR for the detection of intracellular persisting Chlamydia trachomatis in synovial fluid depending on DNA extraction method

From: Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems

Number of serial dilution 1 Alkaline lysis 2 Qiaex II + CTAB gel extraction kit® 3 Qiagen Tissue Kit® 5 Qiagen Stool Kit®
1 1 0.1 10 1
2 0.1 10 100 1
3 0.1 100 1 1
4 1 1 10 1
5 0.1 10 10 1
Sensitivity 0.1 M 0.1 10 M 10 10 M 10 1 M 1
Statistical analysis * 2, 4 * 1 * 2, 5 * 4
  1. Synovial fluid was spiked with C. trachomatis persistently infected peripheral blood monocytes (C. trachomatis PBMO) per ml synovial fluid (SF) ranging from 10,000 C. trachomatis PBMO/ml SF to 0.1 C. trachomatis PBMO/ml SF in 10-fold decreasing numbers. Five independent repeats of each serial dilution were performed (n = 5) for each DNA extraction method. Amplification was performed using system number 1 (C. trachomatis-omp1 directed PCR). The median (M) of serial dilutions is given below. Sensitivity was defined as reproducibly detected lowest number of detected C. trachomatis PBMO/ml SF. Statistical significant results are indicated by *, the method compared with is indicated by number (P < 0.05). omp-1 = major outer membrane protein.