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Table 4 PCR sensitivities of the different DNA extraction methods detection Chlamydia trachomatis EB and C. trachomatis PBMO DNA/ml SF using PCR-system 1 for amplification immediately after extraction and post storage at -20°C for four months

From: Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems

Sensitivity achieved with PCR amplification system 1 1 Alkaline lysis immediately 1 Alkaline lysis ps 2 Qiaex II gel extraction kit® +CTAB immediately 2 Qiaex II gel extraction kit® + CTAB ps 4 Qiagen Tissue Kit® immediately 4 Qiagen Tissue Kit® Ps 5 Qiagen Stool Kit® immediately 5Qiagen Stool Kit® ps
(C. trachomatis EB/ml SF) (0.1 -10) M 1 10 (1-10) M 10 0.1 1000 (1000) M 1000 1 (1-10) M 1 10 (1-10) M 10 1 (1-100) M 100 1 (1-10) M 10
Statistical analysis ps See Table 3 * See Table 3 * See Table 3   See Table 3  
(C. trachomatis PBMO/ml SF) (0.1-1) M 0.1 1000 (1000) M 1000 10 (0.1 - 100) M 10 1000 (1000) M 1000 10 (1-100) M 10 1000 (1000) M 1000 1 (0.1-1) M 1 1000 (1000) M 1000
Statistical analysis ps See Table 4 * See Table 4 * See Table 4 * See Table 4 *
  1. Statistical analysis post storage (ps) compared with sensitivity results compared with sensitivity achieved after immediate PCR analysis are indicated by *. EB = elementary bodies; M = median; PBMO = peripheral blood monocytes; SF = synovial fluid.