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Figure 5 | Arthritis Research & Therapy

Figure 5

From: CTLA4-Ig interacts with cultured synovial macrophages from rheumatoid arthritis patients and downregulates cytokine production

Figure 5

Reverse transcriptase-polymerase chain reaction and western blot analysis for IL-6, TNFα and TGFβ expression in co-cultures of macrophage/Jurkat. Reverse transcriptase-polymerase chain reaction assay in co-cultures of macrophage/Jurkat, untreated (line 1: control) and treated with CTLA4-Ig 10 μg/ml (line 2), CTLA4-Ig 100 μg/ml (line 3), CTLA4-Ig 500 μg/ml (line 4), for (a) β-actin (internal positive control), (b) IL-6, (c) TNFα and (d) TGFβ. Line 5: molecular weight. Densitometry analysis of western blot in co-cultures of macrophage/Jurkat, untreated (line 2: control) and treated with CTLA4-Ig 10 μg/ml (line 3), CTLA4-Ig 100 μg/ml (line 4), CTLA4-Ig 500 μg/ml (line 5) for (e) IL-6 and (f) TNFα protein expression in co-cultures of macrophage/Jurkat. Line 1: molecular weight.

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