IL-23 is critical for the induction of IL-17A and RORγt in TCRγδ T cells. A and B. At days 1 and 7 of AIA, splenocytes were isolated and stimulated for four hours with PMA/Ionomycin, gated for CD3+TCRγδ+ T cells and analyzed for intracellular IL-17A and IFN-γ respectively. Numbers in quadrants indicate percentage of cytokine-positive cells. C and D. Quantification of flow cytrometric analyses from A and B for IL-17A and IFN-γ; mean values and SEM are given for three to six mice for day 1 and for nine mice for day 7. E. Cell counts of total amount of TCRγδ+ T cells present in spleen on day 1 and 7 of AIA in WT and IL-23p19KO mice. F. On day 7 of AIA, splenic CD3+TCRγδ+ T cells were FACS-sorted and IL-17F gene expression was analyzed by quantitative RT-PCR. Mean values and SEM are given for three mice. P-values were calculated using the student's t-test.