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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Interleukin-23 is critical for full-blown expression of a non-autoimmune destructive arthritis and regulates interleukin-17A and RORγt in γδ T cells

Figure 3

IL-23 is critical for the induction of IL-17A and RORγt in TCRγδ T cells. A and B. At days 1 and 7 of AIA, splenocytes were isolated and stimulated for four hours with PMA/Ionomycin, gated for CD3+TCRγδ+ T cells and analyzed for intracellular IL-17A and IFN-γ respectively. Numbers in quadrants indicate percentage of cytokine-positive cells. C and D. Quantification of flow cytrometric analyses from A and B for IL-17A and IFN-γ; mean values and SEM are given for three to six mice for day 1 and for nine mice for day 7. E. Cell counts of total amount of TCRγδ+ T cells present in spleen on day 1 and 7 of AIA in WT and IL-23p19KO mice. F. On day 7 of AIA, splenic CD3+TCRγδ+ T cells were FACS-sorted and IL-17F gene expression was analyzed by quantitative RT-PCR. Mean values and SEM are given for three mice. P-values were calculated using the student's t-test.

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