Figure 4

IL-23 is essential for RORγt expression in TCRγδ ⁺ T cells. At day 7 of AIA, WT and IL-23p19KO mice were sacrificed and splenocytes were isolated. A. CD3⁺CD4⁺ T cells were FACS-sorted and RORγt gene expression was analyzed by quantitative RT-PCR (left panel); RORγt was measured intracellular and CD4⁺ T cells were gated (right panel). B T-bet expression was measured intracellular and CD4⁺ T cells were gated. C. CD3⁺TCRγδ⁺ T cells were FACS-sorted and RORγt gene expression was analyzed by quantitative RT-PCR (left panel); RORγt was measured intracellular and γδ⁺ T cells were gated (right panel). D. T-bet expression was measured intracellular and γδ⁺ T cells were gated. E. Comparison of the mRNA quantification of RORγt expression between FACS-sorted CD4⁺ and γδ⁺ T cells. Mean values and SEM are given for three mice and P-values were calculated using the student's t-test.