IL-23 deficiency results in less IL-17 production in the inflamed joint. At day 7 of AIA, cells from the arthritic joints of WT and IL-23p19KO mice were isolated and stimulated for four hours with PMA/Ionomycin. A. Intracellular cytokine staining of TCRγδ+ T cells for IL-17. B. Intracellular cytokine staining of TCRγδ+ T cells for IFN-γ; data from A and B are representatives from three mice per group. C. Quantification of flow cytrometric analyses from A and B. D. MFI was calculated for TCRγδ+ IL-17+ T cells. E. MFI was calculated for TCRγδ+ IFN-γ+ T cells. F. Total numbers of TCRγδ+ T cells in the joints of arthritic WT and IL-23p19KO mice. C-F, Mean values and SEM are given for three mice and P-values were calculated using the student t-test. G. Intracellular cytokine staining of CD4+ T cells for IL-17A and IFN-γ. Data are representatives of six mice per group. H. Quantification of flow cytrometric analyses from G. I. Total numbers of CD4+ T cells present in arthritic joints from WT and IL-23p19KO mice.