Clinical score time trend. The severity of disease evolution over time was analyzed using Spearman rank correlation. We correlated clinical scores with the age of the mice, expressed in weeks, and divided the study into two periods of time. (a) Correlation between week 21 and week 32 for all of the immunized mice (n = 15). We observed an aggravation of disease in all mice immunized with tumor necrosis factor kinoid (TNF-K) a couple of weeks after the last immunization. (b) Correlation between week 33 and week 45 for immunized mice not receiving the maintenance dose (md). We observed an aggravation of the severity of the disease. (c) Correlation between week 33 and week 45 for immunized mice receiving the maintenance dose. After the maintenance dose at 32 weeks of age, we observed an amelioration of the scores. (d) Correlation between week 28 and week 45 for infliximab-treated mice. The injections were stopped at week 27, and we observed an aggravation of the disease over time thereafter. CI, confidence interval.