Table 1 Human oligonucleotide primer sequences used for RT-PCR
Primer Name | Forward Primer | Reverse Primer | Amplicon Size (bp) | Genbank Accession Number |
|---|---|---|---|---|
GAPDH | GCCTCCTGCACCACCAAC | GAGCTTGACAAAGTGGTCG | 482 | [Genbank: NM_002046] |
SemaA | GAGACTTGGTATGATTTAGAAG | GCTCTGTGTCAATGACTTCC | 618 | [Genbank: NM_006080] |
SemaF | AGTGTCCGTACGATCCCAAG | CAACAGTGACCACCGTCATC | 328 | [Genbank: NM_004186] |
NRP-1 | CTCCTGTTGTGTCTTCAGG | CCCGATGAGGATCGGATTC | 396 | [Genbank: NM_003873] |
NRP-2 | CATGCACTATGACACCCCTG | ATGGGTTCCATGCAGTTCTC | 756 | [Genbank: NM_201266] |
PLXA1 | GACTTCCTGCTGACCCTGAG | GACTTCAACCTGAAGCCAGC | 689 | [Genbank: NM_032242] |
PLXA2 | GCTACAAGAGCTGGGTGGAG | CTCTCGGCTTGAAGAACCAC | 538 | [Genbank: NM_025179] |
PLXA3 | CAGCAGATCGACTACAAGAC | GCCGTGTCAGGTAGATCTC | 564 | [Genbank: NM_017514] |
PLXA4 | TGTCAGGTGTCAACGAGAGC | ATACACCTGCTCCTTGGTGG | 350 | [Genbank: NM_020911] |
PGP9.5 | TGCTGAACAAAGTGCTGTCC | AAAGGCATTCGTCCATCAAG | 508 | [Genbank: NM_004181] |