Table 1 Data distribution and statistical analysis
Fold change of the respective patient sample in the control group (log2) | ||||||
|---|---|---|---|---|---|---|
Experiment | Group | Numbera | 25th quartile | Median | 75th quartile | P value |
MTT assay | B: 1 ng/mL TNF-α | 36 | 0.37 | 0.60 | 0.85 | B vs. A: <0.001 |
C: 10 ng/mL TNF-α | 68 | 0.43 | 0.60 | 0.85 | C vs. A: <0.001 | |
D: 1 ng/mL TNF-α + 10 μg/mL IFX | 36 | -0.03 | 0.13 | 0.39 | D vs. B: <0.001 | |
E: 10 ng/mL TNF-α + 10 μg/mL IFX | 36 | 0.21 | 0.38 | 0.78 | E vs. C: <0.001 | |
F: 10 μg/mL IFX | 36 | -0.21 | 0.02 | 0.30 | F vs. A: 0.2 | |
G: 10 ng/mL TNF-α + 10 μg/mL Diclo | 28 | 0.02 | 0.16 | 0.30 | G vs. C: 0.031 | |
3D collagen gel | B: 1 ng/mL TNF-α | 40 | -0.01 | 0.23 | 0.46 | B vs. A: <0.001 |
C: 10 ng/mL TNF-α | 52 | 0.71 | 0.90 | 1.47 | C vs. A: <0.001 | |
D: 1 ng/mL TNF-α + 10 μg/mL IFX | 40 | -0.44 | -0.22 | 0.04 | D vs. B: <0.001 | |
E: 10 ng/mL TNF-α + 10 μg/mL IFX | 40 | -0.35 | -0.14 | 0.19 | E vs. C: <0.001 | |
F: 10 μg/mL IFX | 40 | -0.23 | -0.01 | 0.15 | F vs. A: 0.6 | |
G: 10 ng/mL TNF-α + 10 μg/mL Diclo | 40 | 0.04 | 0.17 | 0.67 | G vs. C: 0.002 | |
qPCR | ||||||
α-SMA | B: 1 ng/mL TNF-α | 8 | -1.01 | -0.14 | 1.15 | B vs. A: 0.8 |
C: 10 ng/mL TNF-α | 25 | -6.29 | -5.64 | -3.48 | C vs. A: <0.001 | |
D: 1 ng/mL TNF-α + 10 μg/mL IFX | 8 | -1.20 | -0.004 | 0.90 | D vs. B: 0.4 | |
E: 10 ng/mL TNF-α + 10 μg/mL IFX | 8 | -0.63 | -0.50 | 0.10 | E vs. C: 0.01 | |
F: 10 μg/mL IFX | 8 | -0.45 | -0.32 | -0.12 | F vs. A: 0.5 | |
G: 10 ng/mL TNF-α + 10 μg/mL Diclo | 9 | -4.85 | -4.32 | -3.74 | G vs. C: 0.015 | |
Collagen type I | B: 1 ng/mL TNF-α | 8 | -0.36 | -0.007 | 0.59 | B vs. A: 1.0 |
C: 10 ng/mL TNF-α | 17 | -4.35 | -3.47 | -3.00 | C vs. A: 0.01 | |
D: 1 ng/mL TNF-α + 10 μg/mL IFX | 8 | -0.93 | -0.30 | 0.54 | D vs. B: 0.2 | |
E: 10 ng/mL TNF-α + 10 μg/mL IFX | 8 | -1.03 | -0.20 | 0.50 | E vs. C: 0.01 | |
F: 10 μg/mL IFX | 8 | -1.60 | -0.009 | 0.27 | F vs. A: 0.6 | |
G: 10 ng/mL TNF-α + 10 μg/mL Diclo | 9 | -2.21 | -1.84 | -0.93 | G vs. C: 0.015 | |