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Figure 1 | Arthritis Research & Therapy

Figure 1

From: RNAi-mediated CD40-CD154 interruption promotes tolerance in autoimmune arthritis

Figure 1

Knockdown of CD40 reduces allogenicity in DCs. DBA-derived DCs were cultured as described in Materials and methods. DCs were transfected with CD40 siRNA using Genesilencer. Forty-eight hours after gene silencing, reduced gene expression of CD40 was detected by RT-PCR a) and flow cytometry b), respectively, without affecting CD80 gene expression determined by flow cytometry c). CD40-silenced DCs were also used to co-culture allogeneic (BALB/c) T cells. T cell proliferation was assessed in MLR d). Data are presented as mean ± SEM. Results represent one of three experiments. * = P < 0.05 versus control siRNA.

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