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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis

Figure 4

Influence of the isolation procedure on the detection of stromal markers. (a) Dot plot showing CD34- allophycocyanin (APC) and isotype staining of viable CD45-positive CD34-enriched cord blood mononuclear cells (CB-MNCs) before and after exposure to the digestion method. (b) Plots showing (top panel) human leucocyte antigen (HLA)-DR- phycoerythrin (PE) staining and (lower panel) forward scatter (FSC)/side scatter (SSC) of viable, CD45-positive peripheral blood mononuclear cells (PBMCs) before and after exposure to the digestion method. (c) Dot plots showing CD73-PE, CD105- fluoro-isothiocyanate (FITC) and isotype stainings of cultured passage five synovium-derived mesenchymal stem cells. (d) Plots showing CD146-PE and isotype staining in viable (negative for 7-aminoactinomycin (7-AAD) in peridinin chlorophyll protein channel) human osteosarcoma cells before and after exposure to the digestion method.

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Arthritis Research & Therapy

ISSN: 1478-6362

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