Figure 5

Effect of glucosamine (GlcN) and NAPA on inhibitor κB kinase alpha (IKKα) nuclear translocation in human primary chondrocytes. The analysis was performed by Western blot. Cells were untreated (CTL), treated with tumor necrosis factor-alpha (TNFα) or pre-treated with 10 mM GlcN (G10) or NAPA (N10) and then stimulated with TNFα for 1 hour. (a) Cytosolic extract probed with antibodies against IKKα and β-actin. (b) Nuclear extract probed with antibodies against IKKα and fibrillarin. *P ≤ 0.05. Results are expressed as fold change with respect to control. A.U., arbitrary units; NAPA, 2-(N-Acetyl)-L-phenylalanylamido-2-deoxy-β-D-glucose.