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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Regulation of microsomal prostaglandin E2synthase-1 and 5-lipoxygenase-activating protein/5-lipoxygenase by 4-hydroxynonenal in human osteoarthritic chondrocytes

Figure 4

Hydroxynonenal-induced leukotriene B 4 biosynthesis and 5-lipoxygenase and 5-lipoxygenase-activating protein expression in human osteoarthritis chondrocytes. Cells were treated with single addition of 10 μM 4-hydroxynonenal for different incubation time periods, as indicated above. (a) Leukotriene B4 (LTB4) production was evaluated by enzyme immunoassay commercial kit. (b) 5-lipoxygenase-activating protein (FLAP) and (c) 5-lipoxygenase (5-LOX) mRNA expression was measured by real-time RT-PCR. Data are means ± standard errors of the mean, n = 5, expressed as percentages of untreated cells. Student's unpaired t test: *P < 0.05, **P < 0.01, ***P < 0.001.

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