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Table 5 NP vs AC and AF microarray comparison

From: Transcriptional profiling of bovine intervertebral disc cells: implications for identification of normal and degenerate human intervertebral disc cell phenotypes

Gene description Gene symbol AC mean AF mean AC&AF mean NP mean Fold change (NP/AC&AF)
keratin 8 KRT8 0.09 0.39 0.28 714.79 2544.79
keratin 18 KRT18 0.01 0.04 0.05 94.10 1860.77
ras homolog gene family, member B RHOB 0.24 0.04 0.40 383.01 968.68
hypothetical protein BC012029 LOC152573 0.05 0.18 0.26 230.02 897.02
cadherin 2, type 1, N-cadherin CDH2 0.09 0.22 0.21 180.47 840.69
Kruppel-like factor 6 KLF6 0.13 0.03 0.12 55.84 456.37
plakophilin 2 PKP2 0.13 0.48 0.37 77.71 212.23
related RAS viral (r-ras) oncogene homolog RRAS 0.74 0.39 0.61 93.40 152.49
synaptosomal-associated protein, 25 kDa SNAP25 0.55 0.81 0.69 94.22 136.76
sclerostin domain containing 1 SOSTDC1 0.28 0.53 0.68 79.27 116.29
optineurin OPTN 0.22 0.36 0.80 88.35 109.86
tropomyosin 2 (beta) TPM2 18.99 94.17 78.02 0.74 -104.73
integrin, alpha 9 ITGA9 38.74 62.19 66.25 0.50 -131.50
pleiotrophin (heparin binding growth factor 8) PTN 208.08 54.11 167.82 1.10 -152.58
Tissue factor pathway inhibitor 2 TFPI2 1080.55 5089.17 4138.76 26.91 -153.83
guanine nucleotide binding protein (G protein), gamma 11 GNG11 0.57 331.31 298.19 1.65 -181.06
podocalyxin-like PODXL 0.50 297.24 208.74 1.14 -182.62
proteoglycan 4 PRG4 917.06 27.09 722.28 3.25 -222.06
a disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 4 ADAMTS4 115.72 2187.60 1561.34 5.25 -297.16
phenylalanine hydroxylase PAH 195.83 0.24 98.50 0.32 -307.92
interferon, gamma-inducible protein 16 IFI16 0.28 61.31 67.55 0.20 -337.80
retinoic acid receptor responder 1 RARRES1 974.75 156.02 671.46 1.98 -338.73
regulator of G-protein signalling 5 RGS5 5.68 436.91 264.83 0.67 -393.62
integrin-binding sialoprotein IBSP 300.90 0.06 159.69 0.37 -433.73
complement factor H CFH 1.72 60.74 86.66 0.12 -729.93
fibulin 1 FBLN1 199.29 0.22 108.88 0.13 -826.03
Serpin peptidase inhibitor, clade F, member 1 SERPINF1 17.15 512.43 281.58 0.34 -830.27
Keratocan KERA 0.05 346.47 341.38 0.38 -893.66
collagen, type X, alpha 1 COL10A1 1192.84 7.19 741.87 0.79 -944.44
Endomucin EMCN 0.45 281.94 189.76 0.16 -1166.81
secreted frizzled-related protein 2 SFRP2 9.98 153.89 106.73 0.08 -1391.84
peptidase inhibitor 15 PI15 478.32 83.74 308.94 0.10 -3231.26
myosin, heavy chain 11, smooth muscle MYH11 1.15 462.12 263.70 0.03 -7708.90
chemokine (C-X-C motif) ligand 1 CXCL1 3.14 549.84 492.06 0.02 -28150.12
  1. NP specific marker genes. Genes identified by NP vs AC and AF microarray comparison. Mean fluorescence intensity is shown for each cell type (AC, AF and NP) along with the mean combined intensity for AC and AF cells and the calculated fold change values (positive and negative) between NP and AC and AF cells that were greater than 100. Highlighted in bold are genes that were taken forward for qRT-PCR and italicised are genes that were identified in previous studies [23,24]. AC, articular cartilage; AF, annulus fibrosus; NP, nucleus pulposus; qRT-PCR, quantitative real time polymerase chain reaction.