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Table 1 Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells

From: Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells

Gene Primer sequences (5' to 3')
Aggrecan Forward: CTG CTT CCG AGG CAT TTC
  Reverse: GCT CGG TGG TGA ACT CTA GG
Type I collagen Forward: AGG GTC CCA ACG AGA TCG AGA TCC
  Reverse: TAC AGG AAG CAG ACA GGG CCA ACG TCG
Type II collagen Forward: GCC TGG TGT CAT GGG TTT
  Reverse: GTC CCT TCT CAC CAG CTT TG
Sox-9 Forward: AGG TGC TCA AAG GCT ACG AC
  Reverse: GCT TCT CGC TCT CGT TCA GA
Type X collagen Forward: AAT GCC CAC AGG CAT AAA AG
  Reverse: AGG ACT TCC GTA GCC TGG TT
IGFBP-3 Forward: CGC CAG GAA ATG CTA GTG AG
  Reverse: ACG GCA GGG ACC ATA TTC T
IGFBP-5 Forward: GAG AAA GCC CTC TCC ATG TG
  Reverse: TCA CGG GAG TCT CTC TCG AT
Noggin Forward: ATC GAA CAC CCA GAC CCT ATC
  Reverse: TCT AGC CCT TTG ATC TCG CTC
CBFA-1 (RUNX2) Forward: GTG GAC GAG GCA AGA GTT TCA
  Reverse: CAT CAA GCT TCT GTC TGT GCC
β-Actin Forward: GAT CAT TGC TCC TCC TGA GC
  Reverse: GTC ATA GTC CGC CTA GAA GCA T
  1. Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells maintained in micromass cultures in the absence and presence of pentosan polysulfate for 7 and 10 days. IGFBP, insulin-like growth factor binding protein.