Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells

Table 1 Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells

Gene	Primer sequences (5' to 3')
Aggrecan	Forward: CTG CTT CCG AGG CAT TTC
	Reverse: GCT CGG TGG TGA ACT CTA GG
Type I collagen	Forward: AGG GTC CCA ACG AGA TCG AGA TCC
	Reverse: TAC AGG AAG CAG ACA GGG CCA ACG TCG
Type II collagen	Forward: GCC TGG TGT CAT GGG TTT
	Reverse: GTC CCT TCT CAC CAG CTT TG
Sox-9	Forward: AGG TGC TCA AAG GCT ACG AC
	Reverse: GCT TCT CGC TCT CGT TCA GA
Type X collagen	Forward: AAT GCC CAC AGG CAT AAA AG
	Reverse: AGG ACT TCC GTA GCC TGG TT
IGFBP-3	Forward: CGC CAG GAA ATG CTA GTG AG
	Reverse: ACG GCA GGG ACC ATA TTC T
IGFBP-5	Forward: GAG AAA GCC CTC TCC ATG TG
	Reverse: TCA CGG GAG TCT CTC TCG AT
Noggin	Forward: ATC GAA CAC CCA GAC CCT ATC
	Reverse: TCT AGC CCT TTG ATC TCG CTC
CBFA-1 (RUNX2)	Forward: GTG GAC GAG GCA AGA GTT TCA
	Reverse: CAT CAA GCT TCT GTC TGT GCC
β-Actin	Forward: GAT CAT TGC TCC TCC TGA GC
	Reverse: GTC ATA GTC CGC CTA GAA GCA T

Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells maintained in micromass cultures in the absence and presence of pentosan polysulfate for 7 and 10 days. IGFBP, insulin-like growth factor binding protein.

ISSN: 1478-6362