Figure 8
Effect of intra-articular administration of IL-17 on cartilage destruction. Naive C57Bl/6 mice were intra-articulately injected into the knee joint with recombinant murine IL-17 (100 ng/ml) at days 0 and 3 (b and d). The contralateral joint received an equal volume (6 μl) of saline (a and c). Knee joints were taken for histology 2 days after the last injection. Proteoglycan depletion was analyzed using Safranin O staining. Cartilage depletion was visualized by diminished staining of the matrix. The effect of IL-17 can be seen as the lighter (b and d) rather than the darker staining (a and c) in cartilage. (a) and (b), Original magnification, × 50; (c) and (d), original magnification × 200. P, Patella; F, femur; JS, joint space; C, cartilage.