Figure 3
From: Anabolic and catabolic responses of human articular chondrocytes to varying oxygen percentages
Quantification of MMPs produced by HAC cultured at different oxygen percentages during the Phase I. (A) Real time reverse transcription-polymerase chain reaction analysis of the expression of MMP-1, -2, -9, -13 mRNA by human articular chondrocytes (HAC) cultured in pellets at 19% and 5% O2. Levels are expressed as fold of difference from ribosomal 18S. (B) Quantification of MMP-1, -2, -9, -13 released in the culture medium. Levels are normalized to the amount of DNA measured in relative pellets. Values are mean ± SD of measurements obtained from three independent experiments. * = significantly different from the 19% O2.