Figure 2From: Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritisEffect of Robo3 on RASF migration and aggressiveness. (a) Transfection of RASF cells (late passage) with an expression plasmid for Robo3 increased migration of rheumatoid arthritis synovial fibroblasts (RASF; n = 4) in the Boyden Chamber model to about 220% compared with control (* P < 0.05). (b) In vitro assays on cartilage destruction revealed significant induction of free glycosaminoglycans after treatment with Robo3-transfected RASF (late passages; n = 3) compared with mock-transfected cells (** P < 0.01). (c) Normal skin fibroblasts (n = 2) expressing Robo3 after transfection resulted in the release of significantly more free glycosaminoglycans than control transfected cells (* P < 0.05). (d) Matrix metalloproteinase (MMP) 1 (n = 3) and MMP3 (n = 4) mRNA expression was quantified by real-time (RT)-PCR, showing significant induction of MMP1 (* P < 0.05) and high induction of MMP-3 in mRobo3 transfected RASF (late passages) compared with control. (e) MMP1 (n = 3) and MMP3 (n = 4) protein expression from the supernatant released from SF in the GAG assay was quantified via ELISA, showing high induction of MMP-1 and of MMP3 in mRobo3 transfected RASF (late passages) compared to control (* P < 0.05).Back to article page