Figure 5

Skeletal muscle expression of Grp75 and calreticulin after lipopolysaccharide injection in mice and rabbits. (a) Representative western blots of tibialis anterior muscle of adult mice, left untreated or 48 hours after a single injection of lipopolysaccharide (LPS), labeled with antibodies for Grp75 and calreticulin (CRT). Staining of α-actinin is shown as a reference for sample loading. (b) Histograms showing mean and standard error of the mean (SEM) for the normalized amount of muscle Grp75 levels of untreated and LPS-treated mice. *Significant difference versus untreated muscles (P < 0.01). (c) Representative western blots of hindlimb muscles of adult rabbits, left untreated or 48 hours after a single injection of LPS, labeled with antibodies for Grp75 and CRT. Staining of α-actinin is shown as a reference for loading. (d) Histograms showing mean and SEM for the normalized amount of muscle Grp75 levels of untreated and LPS-treated rabbits. *Significant difference versus untreated muscles (P < 0.05). (e) to (h) Distribution of Grp75 immunoreactivity with indirect immunoperoxidase in hindlimb muscles of (e) untreated and (g) LPS-treated rabbits. Adjacent sections (f) and (h) allow the comparison with the distribution of type 1 fibers, identified by the immunoperoxidase staining for β-myosin heavy chain (βMy). Bar: 50 μm.