Table 2 The rearranged heavy chain variable segment genes, their functionality, respective germline gene (homology), ratio of mutations leading to amino acid replacement to silent mutations with the number of mutations for the complementarity determining region and the framework region, heavy chain joining segments and CDR3 lengths
VH | Gene | Functionality | Germline | Homology (%) | R:S ratio CDR | R:S ratio FR | JH | CDR3 (base pairs) |
|---|---|---|---|---|---|---|---|---|
1 | F5-3b6 | nf | DP6 | 97 | 0 | 2/0 | 6 | 26 |
F5-3a3 | pf | DP7 | 91 | 4/0 | 4/4 | 3 | 24 | |
F5-2c2 | pf | DP14 | 100 | 0 | 0 | 2 | 30 | |
F5-2c6 | pf | DP14 | 99 | 0 | 0 | 6 | 30 | |
2c-3m4b | pf | DP25 | 89 | 5/6 | 3/10 | 3 | 39 | |
F5-5c6 | pf | DP25 | 92 | 9/1 | 4/2 | 6 | 39 | |
F5-2c4* | pf | DP21 | 99 | 0 | 2/1 | 1 or 2 or 4 | 21 | |
3a-5m3 | pf | DP21 | 100 | 0 | 1/0 | 5 | 18 | |
F5-3a6 | pf | DP10 | 98 | 2/0 | 1/0 | 6 | 36 | |
5c-4m4 | pf | DP10 | 88 | 9/2 | 11/7 | 4 or 5 | 18 | |
F5-3a2 | pf | DP75 | 97 | 2/1 | 3/1 | 1 | 27 | |
F5-5c1 | pf | DP75 | 98 | 0/0 | 2/0 | 1 | 30 | |
F5-5c2 | nf | DP75 | 96 | 1/2 | 2/0 | Undetermined | 23 | |
5c-4m7 | pf | DP75 | 93 | 6/2 | 4/4 | 4 | 30 | |
2 | 2c-VH24s | pf | HUMIGVH2e | 97 | 1/1 | 2/0 | 4 | 45 |
5c-VH22s | pf | HUMIGVH2e | 99 | 1/1 | 0/0 | 2 | 36 | |
5c-VH26s | pf | HUMIGVH2f | 95 | 2/1 | 2/5 | 6 | 15 | |
3 | 2c-17m14 | pf | DP54 | 95.5 | 3/2 | 4/2 | 2 or 5 | 30 |
2c-19m8 | pf | DP54 | 95.5 | 4/1 | 3/2 | 4 | 12 | |
3a-19m6 | pf | DP54 | 95.5 | 3/0 | 5/2 | 4 | 12 | |
3b-18m4 | nf | DP54 | 100 | 0/0 | 0/1 | 3 | 23 | |
5c-21m1 | nf | DP54 | 96.7 | 5/0 | 1/0 | 5 or 6 | 22 | |
2c-18m7 | pf | DP47/V3-23 | 92.4 | 8/2 | 2/4 | 3 | 30 | |
2c-21m2 | pf | DP47/V3-23 | 96.9 | 3/0 | 2/2 | 6 | 33 | |
2c-21m3 | pf | DP47/V3-23 | 90.1 | 7/6 | 3/3 | 6 | 45 | |
3a-19m8 | pf | DP47/V3-23 | 93.3 | 3/2 | 6/4 | 4 | 24 | |
F5-3a21 | pf | DP47/V3-23 | 92.9 | 2/0 | 4/2 | 5 or 6 | 45 | |
5c-19m9 | pf | DP47/V3-23 | 95.5 | 3/3 | 4/0 | 4 | 33 | |
5c-21m5 | pf | DP47/V3-23 | 88.4 | 10/1 | 8/6 | 6 | 27 | |
F5-5c18 | pf | DP47/V3-23 | 91.2 | 6/1 | 3/3 | 3 | 24 | |
3b-17m1 | pf | DP46 | 96.9 | 2/1 | 2/1 | 2 | 42 | |
F5-3b21 | pf | DP46 | 95.9 | 0/1 | 1/1 | 6 | 21 | |
2c-20m10 | nf | DP50 | 85.7 | 5/2 | 10/0 | 5 | 26 | |
3a-16m4 | pf | DP50 | 95.8 | 4/1 | 0/3 | 1 | 36 | |
3b-17m6 | pf | DP50 | 93.3 | 7/2 | 4/2 | 2 or 4 | 36 | |
5c-19m7 | nf | DP50 | 87.8 | 7/2 | 5/1 | 4 | 31 | |
5c-20m8 | pf | DP50 | 91.1 | 9/3 | 5/3 | 5 | 24 | |
2c-17m9 | pf | DP49 | 88.8 | 3/4 | 8/10 | 2 | 6 | |
2c-20m9 | pf | DP49 | 93.8 | 5/1 | 6/2 | 5 | 24 | |
5c-19m4 | nf | DP49 | 94.0 | 5/3 | 5/0 | 4 | 30 | |
2c-20m8 | pf | DP51 | 98.2 | 0/0 | 3/1 | 5 | 27 | |
3b-16m4 | pf | DP51 | 93.3 | 6/2 | 4/3 | 1 | 27 | |
5c-20m6 | nf | DP51 | 90.2 | 13/2 | 1/6 | 5 | 29 | |
2c-19m2 | pf | DP31 | 97.8 | 2/2 | 1/0 | 4 | 18 | |
F5-3a17 | pf | DP31 | 88 | 5/2 | 2/1 | 2 | 36 | |
3a-18m2 | pf | DP31 | 93.6 | 4/0 | 2/1 | 3 | 12 | |
3a-19m2 | pf | DP31 | 97.3 | 2/1 | 0/1 | 4 | 30 | |
2c-18m3 | pf | DP35 | 100 | 0/0 | 0/0 | 3 | 24 | |
2c-19m7 | pf | DP35 | 94.6 | 4/2 | 4/2 | 4 | 33 | |
5c-21m8 | nf | DP35 | 94.6 | 3/1 | 0/5 | 6 | 31 | |
3a-20m7 | pf | DP77 | 88.3 | 12/2 | 9/4 | 5 | 33 | |
F5-5c16 | pf | DP77 | 94.3 | 4/0 | 6/2 | 1 | 27 | |
2c-21m6 | nf | DP52 | 98.2 | 0/0 | 2/0 | 6 | 25 | |
3a-18m5 | pf | DP29 | 89.9 | 6/1 | 9/2 | 3 | 39 | |
5c-21m2 | pf | DP53 | 97.3 | 3/0 | 2/1 | 6 | 12 | |
4 | 3a-VH43b | pf | HUMIGHCAK | 97 | 1/2 | 4/1 | 3 | 27 |
5c-VH44a | pf | HUMIGHCAK | 95 | 8/1 | 3/1 | 6 | 24 | |
3b-VH45a | pf | DP63 | 93 | 4/0 | 7/4 | 4 | 23 | |
5c-VH44c | pf | DP63 | 99 | 0/1 | 0/0 | 4 | 27 | |
5c-VH41a | nf | DP71 | 99 | 0/0 | 3/0 | 1 | 12 | |
5c-VH42c | pf | HUMIGHCAG | 98 | 1/0 | 3/0 | 2 | 30 | |
5c-VH46b | pf | HUMIGHCAG | > 99 | 1/0 | 0/0 | 6 | 45 | |
5 | 2c-VH53b | pf | DP73 | 97 | 3/0 | 4/0 | 3 | 45 |
2c-VH53c | pf | DP73 | 95 | 2/1 | 8/0 | 3 | 48 | |
2c-VH54c | pf | DP73 | 85 | 12/1 | 16/6 | 4 | 33 | |
2c-VH54b | pf | DP73 | 95 | 4/1 | 4/3 | 4 or 6 | 39 | |
3a-Vh53s | pf | DP73 | 96 | 5/0 | 3/4 | 3 | 66 | |
3a-VH54s | pf | DP73 | 100 | 0/0 | 0/0 | 4 | 36 | |
3a-VH56s | nf | DP73 | 90 | 13/1 | 7/0 | 5 or 6 | 31 | |
3b-VH53s | pf | DP73 | 95 | 4/1 | 5/2 | 3 or 5 | 12 |