Figure 4

ID3 associated with disease activity as well as anti-dsDNA and anti-Sm antibodies. a. Expressions of ID genes decreased after OAZ silencing in vitro. Cells collected from silence study were applied for the detection of ID1 to 4 expressions using qPCR. The EIR was obtained for each subject and the inhibition efficacy was then calculated as (1-EIR) ×100%. After OAZ silencing, expression levels of ID1, ID2, ID3 and ID4 were reduced by 68.7%, 70.2%, 67.7% and 43.3%, respectively. b. Elevated ID3 expressions in peripheral blood cells from SLE patients. Each symbol represents an individual sample and horizontal lines show median values. The relative expression levels of ID3 were 0.75 ± 0.09 in SLE patients and 0 ± 0.11 in normal controls (P < 0.0001). c. ID3 expression correlated with SLE disease activity. According to Pearson's correlation test, log 10 values of ID3 expression levels were significantly correlated with SLEDAI scores (r = 0.76, P < 0.0001). d. ID3 expression associated with anti-dsDNA antibody and anti-Sm antibody. Each symbol represents an individual sample. Those with positive anti-dsDNA antibody had higher expression of ID3 in peripheral blood than those without (P < 0.05), as did those with positive anti-Sm antibody (P < 0.05).