Figure 4
Silencing of tumor necrosis factor receptor 1 (TNFR1) in synovial lining cells ameliorates collagen-induced arthritis (CIA). One day after collagen booster, knee joints of CIA-negative mice were injected with 107 ffu hairpin construct targeting TNFR1 (HpTNFR1) or hairpin non specific (HpNS). (a) Expression of indicated genes in synovial tissue at day 26 of CIA. Data are represented as mean ± standard error of the mean (SEM) (n = 6) of the difference in ΔCt values compared with the HpNS group (ΔΔCt). (b) Appearance of arthritis in fore and hind paws was monitored at indicated time points and scored for severity. (c) Histological analysis of inflammation ('infiltrate' and 'exudate') and proteoglycan depletion in patellar and femoral cartilage ('PG loss') from knee joints isolated at day 31. Data are represented as mean ± SEM (n = 9 mice), and statistical differences were calculated using Mann-Whitney U test. *P < 0.05, **P = 0.01. Ct, cycle threshold; IL, interleukin; TNF, tumor necrosis factor.