Figure 4

Time course of 2GF-induced potentiation of (a) IL6, (b) MIP1α and (c) MMP3 RNA induced by TNFα. FLS were cultured for indicated times with TNFα and growth factors, and mRNA levels quantified by real-time qPCR using GAPDH as housekeeper. Data are normalized to levels with TNFα alone at 24 h. Mean & SEM, n = 3 RA FLS lines. Asterisk indicates P < 0.05 to TNF alone and 2GF alone by two-way ANOVA and contrast testing on log-transformed data.